Figure 1

Chilling sensitivity of suspension cells of rice and bromegrass cultured at 4°C in the presence or absence of 75 μM ABA (A) and growth of rice and bromegrass cells during prolonged culture at 4°C with or without 75 μM ABA (B). Rice or bromegrass cells (0.25 g) inoculated in 12.5 mL of N6 medium or ER medium were incubated at 4°C in the presence or absence of 75 μM ABA for designated periods following 2 day pre-incubation at 25°C. Survival of chilled cells was determined by regrowing the cells (non-stressed cells as the 100% control). Values indicated by different letters (a-f) were significantly different (α = 0.05) using Tukey-Kramer multiple comparison analyses (Figure 1B).