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Figure 1 | BMC Research Notes

Figure 1

From: Identifying technical aliases in SELDI mass spectra of complex mixtures of proteins

Figure 1

Examples of mass spectra of purified transthyreting and complex mixtures of proteins. In the upper spectra, purified transthyretin was applied to a CM10 ProteinChip array and spectra at medium (left) and high (right) laser energies were acquired. Peaks for transthyretin with zā€‰=ā€‰1 and 2, and transthyretin dimers are prominent in the medium laser spectrum, while aggregates of 2ā€“9 transthyretin molecules can be seen at high laser energy. The lower spectra are averaged from 90 individual spectra (45 samples in duplicate) of the pH4 extract from Q anion exchange chromatographed plasma samples applied to CM10 ProteinChip arrays. Peaks for Ī±-globin and albumin that were used to develop mass windows for the algorithm are annotated.

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