In vivo DSS exposure completely inhibits qPCR but does not alter RNA integrity. A) Total RNA was extracted from colonic tissues obtained from mice treated with or without 3% DSS, and RNA integrity was assessed by 2% agarose gel electrophoresis. DSS treatment does not alter the integrity of the RNA. B) After cDNA synthesis, qPCR was performed for the 36B4 amplicon. The amplification of 36B4 was totally inhibited in DSS-treated mice. C) The DSS- and non-DSS RNA samples were purified using our lithium chloride protocol, and qPCR was performed. After lithium chloride purification, 36B4 was successfully amplified from both DSS- and non-DSS samples.