Figure 5From: Dextran sodium sulfate inhibits the activities of both polymerase and reverse transcriptase: lithium chloride purification, a rapid and efficient technique to purify RNADSS alters the interaction between primed RNA and reverse transcriptase. Primed RNA was incubated with or without different concentrations of DSS, and binding to the M-MuLV reverse transcriptase was determined by SPR (measured in mDeg). A) The primed RNA binds to the reverse transcriptase, deflecting the resonance angle to 42 mDeg. B-D) After incubation with 0.05 (B), 0.5 (C) or 5 (D) g/L of DSS, the primed RNA binds to the reverse transcriptase, deflecting the resonance angle to 25.6 mDeg. Δt = Δtotal.Back to article page