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Figure 5 | BMC Research Notes

Figure 5

From: Dextran sodium sulfate inhibits the activities of both polymerase and reverse transcriptase: lithium chloride purification, a rapid and efficient technique to purify RNA

Figure 5

DSS alters the interaction between primed RNA and reverse transcriptase. Primed RNA was incubated with or without different concentrations of DSS, and binding to the M-MuLV reverse transcriptase was determined by SPR (measured in mDeg). A) The primed RNA binds to the reverse transcriptase, deflecting the resonance angle to 42 mDeg. B-D) After incubation with 0.05 (B), 0.5 (C) or 5 (D) g/L of DSS, the primed RNA binds to the reverse transcriptase, deflecting the resonance angle to 25.6 mDeg. Δt = Δtotal.

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BMC Research Notes

ISSN: 1756-0500

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