Figure 4

Confirmation of biochemical differentiation of SH-SY5Y. (a) Lysates of SH-SY5Y cells, undifferentiated or differentiated with IGF-1 or RA were run on 12% SDS-PAGE gels and probed for differentiation marker proteins, β3 tubulin and GAP43, followed by detection with LI-COR Odyssey™. Densitometry of protein bands was measured using LI-COR Odyssey™ software and normalised to cellular GAPDH levels. (b) The fold increase in β3 tubulin signal compared to undifferentiated protein level are plotted on a bar chart ± SEM, n = 3. (c) The fold increase in GAP43 signal compared to undifferentiated protein level are plotted on a bar chart ± SEM, n = 3. Significant differences were measured by ANOVA (^#P < 0.05 for comparisons between undifferentiated cells and both differentiation conditions; *P < 0.05 for comparisons between RA differentiated cells and IGF-1 differentiated cells.