Figure 7

Pathways of apoptosis induction in DENV 2 infected HepG2 cells. HepG2 cells were either infected at 10 p.f.u./cell or mock infected and examined at the times indicated by (a, b, c, d) Western blot for the activation of caspases 4, 7, 8 and 12 or (e) by ELISA for activation of caspase 9 (solid bars day 1 p.i., open bars day 2 p.i.). (f) DENV infected HepG2 cells were examined for the expression of TRAIL by RT-PCR and (g) by microscopy after staining with MDC for the presence of autophagic vacuoles. * indicates p < 0.05.