Figure 3

Quantitative real-time (qRT)-PCR expression analysis of full-length and mutant CEACAM5 . The mRNA expression levels of full-length CEACAM5 and novel variants 5D and 3D are shown in the upper panel, middle panel and lower panel, respectively. qRT-PCRs using specific primers was performed with 20 ng of cDNA templates synthesized from extracted mRNAs. Data are expressed as mean value ± S.D. in triplicate experiments.