Figure 4From: Novel protein isoforms of carcinoembryonic antigen are secreted from pancreatic, gastric and colorectal cancer cellsImmunoblot detection of CEA in pancreatic, gastric, and colorectal cancer cells. Cell lysate samples of each cell line (10 μg protein/lane) were analyzed by immunoblotting with the anti-CEA mouse monoclonal antibody (1:3,000 dilution), either directly (A) or after PNGase F treatment (B). PNGase F treatment was undergone to cleave N-glycan chain from proteins. ACTB (actin-ß) was also detected as an internal loading control. The detected bands were separated into three regions, indicated as Regions 1, 2, and 3.Back to article page