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Table 1 Peptide sequences identified by LC-MS/MS analysis

From: Novel protein isoforms of carcinoembryonic antigen are secreted from pancreatic, gastric and colorectal cancer cells

Band region†

Sequence

Motif‡

Region 1

KLTIESTPFNVAEGK

N

QIIGYVIGTQQATPGPAYSGR

N

SDLVNEEATGQFR

N

TLTLFN*VTR

A1/A3§

CETQNPVSAR

A1

SDSVILNVLYGPDAPTISPLN*TSYR

A1-B1

LQLSNDN*R

A2

NSGLYTCQAN*NSASGHSR

B2

LQLSNGN*R

A3

AYVCGIQNSVSAN*R

A3

ITPNNN*GTYACFVSNLATGR

B3

Region 2

KLTIESTPFNVAEGK

N

SDLVNEEATGQFR

N

TLTLFN*VTR

A1/A3§

TLTLLSVTR

A2

Region 3

TLTLFN*VTR

A1/A3§

ITPNNN*GTYACFVSNLATGR

B3

  1. _*, conversion of asparagine to aspartic acid as a result of PNGase F treatment.
  2. †, region detected by immunoblotting described in Figure 4.
  3. ‡, the name of IgC/V-like domains described in Figure 5.
  4. §, the same tryptic peptides on A1 and B3 domains.