Figure 3From: An orange fluorescent protein tagging system for real-time pollen trackingAverage relative fluorescence intensity for TN 90 TD-GFP-H T 1 lines. Leaf tissue from a non-transgenic TN 90 line and ten transgenic T1 lines were excited at 395 nm and measured at an emission of 509 nm with a spectrofluorometer. Fluoresence intensity values were normalized to an average measurement of TN 90 negative control plants outside the spectral range of GFP emission. For TN 90 n = 2, TD-GFP-H lines1-8, and 10 n = 34, line 9 n = 33. Error bars represent the standard error from the mean and different letters indicate significant differences at P < 0.05.Back to article page