Fluorescent protein visualization in pollen. Orange fluorescent protein-tagged pollen as viewed under an epifluorescent microscope at 100×. N. glauca and TN 90 plants have been bred for homozygosity of tdTomato-ER, resulting in hybrid OFP plants. All white light images (A), (C), (E), (G), and (I) were captured at 80 ms exposure time. Panels (B), (D), (F), (H), and (J) are epifluorescent microscopy images using green light excitation and orange light emission. Panels (B) and (D) were captures at 50 ms exposure time. Panels (F) and (H) were captured at 80 ms exposure time. Panel (J) was captured at 180 ms exposure time.