Lateral views from zebrafish insertional mutants for five V-ATPase subunits. Larvae WT (A) and mutants in subunits V0-d1 (B), V0-ac45b (C), V1-E1b (D), V1-H (E) and V0-ca (F) are shown. To the right from every image there is a close up view of the region indicated by the white arrow in the left panel. Melanin round spots accumulate ventrolateral to the ear, which is an identical feature observed in the class VI.C of zebrafish mutants from the Tubingen chemical screening (see reference 38). Even though the reason for this phenotypic characteristic is unknown, it could be used as a form to identify this type of mutations.