Table 2 Primer sequences used for qRT-PCR analysis
Gene name | Primers-(5′-3′) forward/reverse | Amplicon size (bp) | PCR Efficiency ± SD1 |
|---|---|---|---|
EF1alpha | GTGGTTCATGATGATGACCTGGGA/ | 82 | 1.90 ± 0.03 |
TGGTTATGTGGCCTCCAACTCCAA | |||
EIF4A | TGGTGATGAGCACACGGGATGAA/ | 80 | 1.90 ± 0.05 |
TCACGGTGACATGGACCAGAACACTA | |||
ACT2 | CCTTCCTGATATCCACATCACA/ | 103 | 1.85 ± 0.03 |
CCTGAGGTCCTCTTCCAACC | |||
UBCE | TGTCATGGCATCGAAGCGTATCCT/ | 106 | 1.87 ± 0.03 |
TTGCCAATGAAACATGTCCTCGCC | |||
GAPDH | TGTCACCAGTGAAGTCCGTGGAAA/ | 94 | 1.95 ± 0.04 |
AAGAAGGCTATCAAGGCTGCGTCT | |||
TUBA | CTGCAGAATTCAGGTTTGATGGTGC/ | 80 | 2.05 ± 0.05 |
GATACGTGGGTATGGAACAAGGTTGG |