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Table 4 Ovary (second independent assay) reference gene stability analyses of B-2S (sex) and B-12-9 (apo) using GeNorm and NormFinder

From: Selection and validation of reference genes for gene expression analysis in apomictic and sexual Cenchrus ciliaris

Genotype_Tissue type

 

GeNorm - M values

NormFinder_Gene ranking (SD values)

Samples

 
  

ACT2

EF1alpha

EIF4A

GAPDH

TUBA

UBCE

ACT2

EF1alpha

EIF4A

GAPDH

TUBA

UBCE

Ovary (early/tetrad/DOP)

Sex

0.19

0.10

0.09

0.03

0.16

0.03

5 (0.24)

1 (0.01)

1 (0.01)

2 (0.13)

4 (0.23)

3 (0.15)

 

Apo

0.30

0.26

0.33

0.39

0.26

0.45

4 (0.32)

2 (0.25)

1 (0.12)

3 (0.30)

5 (0.44)

6 (0.54)

Ovary (early/tetrad/DOP/DOP + 5)

Sex

0.24

0.16

0.09

0.06

0.29

0.06

4 (0.29)

5 (0.31)

1 (0.05)

2 (0.07)

6 (0.35)

3 (0.12)

 

Apo

0.30

0.30

0.32

0.35

Oc1

0.40

3 (0.23)

2 (0.16)

1 (0.10)

4 (0.28)

6 (0.81)

5 (0.56)

  1. GeNorm stability value is represented by M value (the selected cut off for M value is ≤0.5). NormFinder stability value is represented by SD (standard deviation). There is no cut-off for SD, hence the ranking of genes based on the SD value is used as a measure to evaluate stability (1 stands for most stable, 6 is least stable). The ovary stages are as follows: early stands for early premeiosis, tetrad, DOP for day of pollination, and DOP + 5 is 5 days after day of pollination.
  2. 1Oc refers to value outside the cut-off range.