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Table 3 Reference genes ranked from the most to the least stable considering healthy Macrosteles quadripunctulatus samples

From: Selection of reference genes from two leafhopper species challenged by phytoplasma infection, for gene expression studies by RT-qPCR

Ranking order

BestKeeper (N = 15)

geNorm (N = 17)

Normfinder (N = 17)

 

Gene name

Std dev [± CP]

Gene name

M value

Gene name

Stability value

1

GAPDH

0.82

ATP Syn β *

1.474

ATP Syn β

0.450

2

ATP Syn β

0.91

GAPDH *

1.773

Tropomyosin

0.897

3

18S

1.04

18S

1.968

18S

1.016

4

Tropomyosin

1.06

Tropomyosin

2.104

GAPDH

1.085

5

Actin

1.80

Actin

2.248

Actin

1.552

  1. *Best combination of two genes according with geNorm (average expression stability M = 0.955).
  2. Best combination of two genes according with Normfinder (stability value = 0.587).
  3. In bold geNorm M values > 1.5.