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Table 4 Reference genes ranked from the most to the least stable considering all Macrosteles quadripunctulatus samples

From: Selection of reference genes from two leafhopper species challenged by phytoplasma infection, for gene expression studies by RT-qPCR

Ranking order

BestKeeper (N = 24)

geNorm (N = 29)

Normfinder (N = 29)

 

Gene name

Std dev [± CP]

Gene name

M value

Gene name

Stability value

1

GAPDH

0.79

ATP Syn β *

1.706

ATP Syn β

0.529

2

ATP Syn β

1.12

GAPDH *

1.923

Tropomyosin

0.551

3

18S

1.12

Tropomyosin

2.056

18S

0.691

4

Tropomyosin

1.18

18S

2.237

GAPDH

0.824

5

Actin

2.15

Actin

2.672

Actin

1.275

  1. *Best combination of two genes according with geNorm (average expression stability M = 1.015).
  2. Best combination of two genes according with Normfinder (stability value = 0.435).
  3. In bold geNorm M values > 1.5.
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BMC Research Notes

ISSN: 1756-0500

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