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Table 7 Reference genes ranked from the most to the least stable considering all Euscelidius variegatus samples

From: Selection of reference genes from two leafhopper species challenged by phytoplasma infection, for gene expression studies by RT-qPCR

Ranking order

BestKeeper (N = 29)

geNorm (N = 30)

Normfinder (N = 30)

 

Gene name

Std dev [± CP]

Gene name

M value

Gene name

Stability value

1

18S

0.56

Tropomyosin

1.143

Actin

0.041

2

GAPDH

0.89

GAPDH *

1.220

GAPDH

0.044

3

Tropomyosin

1.18

ATP Syn β *

1.256

ATP Syn β

0.045

4

ATP Syn β

1.24

Actin

1.366

Tropomyosin

0.049

5

Actin

1.27

18S

1.423

18S

0.063

  1. *Best combination of two genes according with geNorm (average expression stability M = 0.759).
  2. Best combination of two genes according with Normfinder (stability value = 0.030).
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BMC Research Notes

ISSN: 1756-0500

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