Selection of reference genes from two leafhopper species challenged by phytoplasma infection, for gene expression studies by RT-qPCR

Table 8 Reference genes ranked by geNorm in Macrosteles quadripunctulatus individuals sampled at different times after acquisition

Ranking order	Time 1-3dpa (N = 13)		Time 2-20dpa (N = 8)		Time 3-40dpa (N = 8)
	Gene name	M value	Gene name	M value	Gene name	M value
1	Tropomyosin ^*	0.826	ATP Syn β ^¶	1.440	*GAPDH* ^§	1.923
2	ATP Syn β	0.903	GAPDH ^¶	1.498	*ATP Syn β* ^§	1.965
3	Actin ^*	0.916	*Tropomyosin*	1.711	*18S*	2.598
4	GAPDH	1.133	*18S*	2.126	*Actin*	2.803
5	*18S*	1.721	*Actin*	2.294	*Tropomyosin*	2.889

^*Best combination of two genes considering individuals sampled at 3 dpa (average expression stability M = 0.394).
^¶Best combination of two genes considering individuals sampled at 20 dpa (average expression stability M = 0.806).
^§Best combination of two genes considering individuals sampled at 40 dpa (average expression stability M = 0.864).
In bold geNorm M values > 1.5.

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