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Figure 1 | BMC Research Notes

Figure 1

From: Vectorial secretion of interleukin-8 mediates autocrine signalling in intestinal epithelial cells via apically located CXCR1

Figure 1

Secretion of IL-8 in polarized Caco-2 BBE intestinal epithelial cells in response to Toll-like receptor ligands. Caco-2 BBE (a) monolayers were stimulated for 24 hours from the apical (Ap treatment) or the basolateral compartment (Bl treatment) with: Pam3CSK4 (20 μg/ml), Pam2CSK4 (10 μg/ml) and flagellin (2 μg/ml). Amounts of IL-8 secreted in the apical (black bars) and basolateral (white bars) compartments are normalized to the volume of supernatant in each chamber. Error bars represent SEM (n=3). (b) IL-8 does not alter trans-epithelial electrical resistance (TER) in Caco-2 BBE monolayers. TER of Caco-2 BBE polarized monolayers untreated (black) or incubated with 100 pg/ml IL-8 on the apical (blue) or the basolateral side (red) for 20 hours. This concentration of IL-8 was chosen because it elicited a differential transcriptional response in Caco-2 BBE cells. TER values were normalized to the initial TER value (100%) and the differences between apical and basolateral treatment and control were not statistically significant. Error bars represent SEM (n=4); *p<0.05, **p<0.01, ***p<0.001.

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