Table 3 Segregation of Basta and kanamycin resistance in T1 plants derived from cotransformed plants
From: A new double right border binary vector for producing marker-free transgenic plants
T0 event | Number of T1 plants assessed by Basta leaf painting | Number of T1 plants analysed by paromomycin Leaf bleach assay | Number of T1 plants | Frequency of generation of marker-free plants | |||||
|---|---|---|---|---|---|---|---|---|---|
BR | BS | KmR | KS | BRKmR | BRKmS | BSKmR | BSKmS | ||
TD56 | 15 | 8 | 15 | 8 | 15 | 0 | 0 | 8 | 0.00% |
TD81 | 15 | 4 | 19 | 0 | 15 | 0 | 4 | 0 | 21.05% |
TD52 | 8 | 11 | 9 | 10 | 6 | 4 | 3 | 6 | 15.79% |