Figure 3

Stability values of reference gene candidates for hCEC qRT-PCR gene expression studies. (A) Average expression stability values of 10 candidate reference genes by stepwise exclusion of least stable genes, using the comparative Ct methods and analysed using the GeNorm algorithm (B) Pair wise variation of candidate gene indicates that 2 reference genes give a low variation so are suitable for normalisation (<0.15), (C) Ct values were transformed into relative quantities using the comparative Ct methods and analysed using the NormFinder algorithm (D) Standard deviation values calculated using BestKeeper, values <1 indicate a suitable housekeeping gene.