Detection of PrPScby Western blotting. WB using P4 antibody of homogenated brain material from animals and inoculation material used in this experiment. Lanes 1–5 (2006 and 2007) represent the scrapie inoculated animals. Lanes 6–10 (2006) and 6–7 (2007) represent the control animals. The lanes indicated by the arrow represent inoculation material used in the scrapie groups. Molecular markers were placed in lanes 12 and 9. PrPSc was detected in inoculation material and in all the animals from the scrapie groups.