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Table 1 Quantitative and qualitative measurements of the total RNA isolated with the 3 protocols

From: Highly efficient method for isolation of total RNA from adipose tissue

Samples Tissue amount Protocol Concentration Ratio 260/280 Ratio 260/230 RQI
560a.fat 100 mg Tri Reagent® 125 ng/μl 1.83 0.85 9.5
560bfat 100 mg Tri Reagent® 205 ng/μl 1.83 0.36 8.3
560a.fat 100 mg miRNeasy 97 ng/μl 1.98 1.39 8.9
560b.fat 100 mg miRNeasy 147 ng/μl 2.01 1.38 8.7
435.fat 100 mg combined 92 ng/μl 1.94 1.46 8.3
473.fat 100 mg combined 115 ng/μl 1.99 1.70 9.1
524.fat 100 mg combined 147 ng/μl 2.02 1.45 8.9
572.fat 100 mg combined 107 ng/μl 1.92 1.57 9.1
584.fat 100 mg combined 107 ng/μl 1.97 1.74 8.6
517a.lean 100 mg Tri Reagent® 109 ng/μl 1.79 0.45 6.4
517b.lean 100 mg Tri Reagent® 89 ng/μl 1.79 0.81 6.3
517a.lean 100 mg miRNeasy 128 ng/μl 2.02 1.78 7.2
517b.lean 100 mg miRNeasy 109 ng/μl 2.05 1.17 2.5
402.lean 100 mg combined 180 ng/μl 2.04 1.69 8.8
407.lean 100 mg combined 125 ng/μl 2.00 1.90 9
470.lean 100 mg combined 185 ng/μl 2.04 1.85 7.7
522.lean 100 mg combined 144 ng/μl 2.05 1.88 8.6
577.lean 100 mg combined 282 ng/μl 2.07 2.01 9.2
  1. Tissue amount: starting amount of tissue used in each protocol; Ratios 260/280 and 260/230 measured by Nanodrop ND-1000; RQI measured by Experion™ machine; fat and lean samples: phenotype of the animals used based on weight and metabolic parameters described elsewhere [2].
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BMC Research Notes

ISSN: 1756-0500

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