Table 3 Sense and antisense primer sequences used in qPCR
Genetic marker | Primer | Sequence | |
|---|---|---|---|
18S Ribosomal RNA gene | Forward | 5'-TAAGTCCCTGCCCTTTGTACACA-3' | |
Reverse | 5'-GATCCGAGGGCCTCACTAAAC-3' | ||
Profilaggrin | FLG | Forward | 5'-TGGCAAATCATCATCTCAAGTG-3' |
Reverse | 5'-CTGTCCTGGCTAACTCTGG-3' | ||
Occludin | OCCL | Forward | 5'-CAAGCGGTTTTATCCAGAGTC-3' |
Reverse | 5'-AAGTCATCCACAGGCGAAG-3' | ||
Claudin1 | CLD1 | Forward | 5'-AGTGCTTGGAAGACGATGAG-3' |
Reverse | 5'-TTGAACGATTCTATTGCCATACC-3' | ||
Transglutaminase 1 | TG1 | Forward | 5'-TGCTGGATGCCTGCTTATAC-3' |
Reverse | 5'-TGCCTCGGGAGTAATCACC-3' | ||
Proliferating cell nuclear antigen | PCNA | Forward | 5'-AACCTCACCAGTATGTCC-3' |
Reverse | 5'-ATCCATCAACTTCATTTCATAG-3’ |