The coding region of the UFGT gene is a source of diagnostic SNP markers that allow single-locus DNA genotyping for the assessment of cultivar identity and ancestry in grapevine (Vitis vinifera L.)

Table 1 List of primers used for each chloroplast and nuclear marker with their chromosome localization, function, amplicon length, primer nucleotide sequences and references

Marker	Localization	Coding region	Length (bp)	Primer name	Primer sequence (5′-3′)	Ta (°C)	References
rps16	Chloroplast	Intron	956	rps_F	GTGGTAGAAAGCAACGTGCGACTT	56	[31]
				rps_R	TGCGGATTCCTAAGAGCAGCGT		[31]
rp132-tmL	Chloroplast	Intergenic spacer	1377	trnLUAGR	CTGCTTCCTAAGAGCAGCGT	50	[32]
				rpl32_F	CAGTTCCAAAAAAACGTACTTC		[32]
trnH-psbA	Chloroplast	Intergenic spacer	460	psbA3′f	GTTATGCATGAACGTAATGCTC	56	[33]
				trnHf	CGCATGGTGGATTCACAATCC		[34]
trnT-tmL	Chloroplast	Intergenic spacer	1016	trnTUGU2F	CAAATGCGATGCTCTAACCT	56	[35]
				5′tmLUAAR	TCTACCGATTTCGCCATATC		[36]
atpB-rbcL	Chloroplast	Intergenic spacer	927	atpB-rbcL_F	AACACCAGCTTTRAATCCAA	56	[37]
				atpB-rbcL_R	ACATCKARKTACKGGACCAATAA		[37]
trnL-trnF	Chloroplast	Intergenic spacer	406	trnL_UNIE	GGTTCAAGTCCCTCTATCCC	50	[36]
				trnL_UNIF	ATTTGAACTGGTGACACGAG		[36]
GAI	Chromosome 1	Transcription factor for GA	761	GAI_F	ATGGATGAGCTTCTGCTGT	50	[27]
				GAI_R	TAGAAGTGCATCCTGRAGAAT		[27]
ID04	Chromosome 3	ZIP DNA-binding protein	419	Id04_F	CACCAGTCCCTTACCAGTCT	55	[38]
				Id04_R	CAGTAGAGGAACACAACTGAG		[38]
IIC08	Chromosome 3	ZINC finger protein	418	IIC08_F	CAAGGCCTTCTCTTCGTACC	60	[38]
				IIC08_R	AAGAATTCATATCGCCGACC		[38]
ATP	Chromosome 7	ATP synthase	800	ATP_F	ATGCTGTTCCAGTCCGTTTC	60	-
				ATP_R	GGGTCGATGGTGATCTTCT		-
UFGT	Chromosome 16	Glucosyltransferase	919	UFGT_F4	ATGTCTCAAACCACCACCAACC	63	-
				UFGT_R3	TGACGGTGCCAAAGCTAATG		-

ISSN: 1756-0500