Table 1 Open reading frames and genetic features of L. sanfranciscensis EV3 phage
From: The genome of the Lactobacillus sanfranciscensis temperate phage EV3
Locus tag | Putative RBS*and start codon‡ | nt | orf length (aa) | Best hit | Best hit EMBL protein name | Score |
|---|---|---|---|---|---|---|
EV3_001 | ttacgaaaggaga aattgtatg | 136-675 | 179 | Phage terminase L. vaginalis, small subunit ATCC 49540 | EEJ41449 | 1.0E-88 |
EV3_002 | gacagtattgctaatatgctaaatg | 650-2590 | 646 | Phage terminase L. vaginalis, large subunit, ATCC 49540 | EEJ41450.1 | 0.0 |
EV3_003 | tgcgataggagg tgattagcaatg | 2786-3964 | 398 | Phage portal protein L. vaginalis ATCC 49540 | EEJ41452.1 | 0.0 |
EV3_004 | taaaggagg tgataatgtaaatg | 3930-5918 | 662 | phage capsid protein L. vaginalis ATCC 49540 | EEJ41453.1 | 0.0 |
EV3_005 | caaaataaaggagt gataaaatg | 5950-6489 | 179 | major tail protein Staph. pseudintermedius HKU10-03 | ADV05789.1 | 1.0E-13 |
EV3_006 | actaagggaga tgagtagcaatg | 6509-6814 | 101 | Putative uncharacterized protein L. vaginalis ATCC 49540 | EEJ41454.1 | 6.0E-45 |
EV3_007 | aacaagggtggtgacagccaatg | 6808-7176 | 122 | Head-tail joining protein L. vaginalis ATCC 49540 | EEJ41455.1 | 5.0E-155 |
EV3_008 | tgtaaaggagc tgagtggcaatg | 7169-7606 | 145 | Head-tail joining protein L. vaginalis ATCC 49540 | EEJ41456.1 | 2.0E-91 |
EV3_009 | aaaggatg ttaaatcatgaaaatg | 7603-7992 | 129 | Phage tail protein L. vaginalis ATCC 49540 | EEJ41424.2 | 7.0E-66 |
EV3_010 | ttagaaaagagg aaataatttatg | 7996-8763 | 255 | Phage major tail protein L. vaginalis ATCC 49540 | EEJ41425.1 | 1.0E-123 |
EV3_011 | cggccattaaggaga taagtaatg | 8873-9283 | 136 | Putative uncharacterized protein L. vaginalis ATCC 49540 | EEJ41426.1 | 9.0E-64 |
EV3_012 | gtcgtaagccaaacgtggctctgatg | 9343-9513 | 56 | Putative uncharacterized protein L. vaginalis ATCC 49540 | EEJ41427.1 | 7.0E-22 |
EV3_013 | gaaggaaggagg taactaaatg | 9514-13305 | 1263 | Phage minor tail protein L. vaginalis ATCC 49540 | EEJ41428.1 | 0.0 |
EV3_014 | aacttaatggagg tcttgcataatg | 13305-14141 | 278 | Putative uncharacterized protein L. saliv arius (strain CECT 5713) | ADJ78578.1 | 3.0E-48 |
EV3_015 | aattttagggagg tgttaatttg | 14161-15396 | 411 | Glycosylhydrolase L. plantarum | CCC78574.1 | 4.0E-17 |
EV3_016 | aggaacaagggtgattatttaatg | 15396-17177 | 593 | Put. Minor structural protein Leu. kimchii IMSNU 11154 | ADG39890.1 | 3.0E-62 |
EV3_017 | tttgtctaggaagg agaaaaaatg | 17190-17726 | 54 | Hypothetical protein | No hit | - |
EV3_018 | aaaagttgggagtgattaaaaatg | 17729-20134 | 801 | Dextranase L. fermentum phage phiPYB5 | ADA798961.1 | 1.0E-137 |
EV3_019 | atgaaataaaggaga aaataaatg | 20212-20721 | 170 | hypothetical protein | No hit | - |
EV3_020 | tggagaaaggagg tgatgaaatg | 20790-21083 | 97 | Predicted protein P. acidilactici | EFA25777.1 | 2.0E-4 |
EV3_021 | aaagaaacgagtgaacaatatg | 21067-21309 | 80 | L. plantarum WCFS1 phage P1 holin, lp0683 | F9ULS1 | 6.0E-68 |
EV3_022 | taccattaatcatcgttgctgaaatg | 21383-22429 | 348 | putative endolysin L. vaginalis ATCC 49540 | EEJ39754.1 | 9.0E-70 |
EV3_023 | aaagcagaaagcgaattaatatg | 23961-22876c | 361 | phage integrase L. salivarius ACS-116-V-Col5a | EFK79983.1 | 1.0E-112 |
EV3_024 | atattctaaggaga atggaaaatg | 24072-24485c | 137 | Putative uncharacterized protein L. pentosus MP-10 | CCB81756.1 | 4.0E-14 |
EV3_025 | tagaatacgattggatattgatatg | 24478-24795c | 110 | XRE family transcriptional regulator L. pentosus | FR871768 | 4.0E-23 |
EV3_026 | tatgaaaaggagg aatcaatatg | 25072-25263 | 63 | Phage antirepressor L. ruminis ATCC 25644 | EFZ34631.1 | 2.0E-10 |
EV3_027 | agaaaaaagatgattgtcatg | 25378-25638 | 89 | Phage protein Listeria monocytogenes FSL N1-017 helix-turn-helix protein | EFK40475.1 | 4.0E-5 |
EV3_028 | tataaggggg tgagatagatg | 25639-25794 | 51 | Hypothetical protein | No hit | - |
EV3_29 | atttcaaggaga tgtaataaatg | 25798-26028 | 77 | Hypothetical protein | No hit | - |
EV3_030 | aatctaaaaggaag ttattcatatg | 26146-26322 | 59 | hydrolase NUDIX family L. delbrueckii | CAI96847 | 0.73 |
EV3_031 | aattttagtggggg tagagaaatg | 26336-26821 | 161 | Putative uncharacterized protein Mahella australiensis DSM 15567 | AEE95754.1 | 6.0E-7 |
EV3_032 | ccgaaaggaag tgagataaatg | 26852-27319 | 155 | Putative uncharacterized protein lp_0862 L. pentosus IG1 | FR874854.1 | 1.0E-22 |
EV3_033 | actaaattataggaga taaatatg | 27380-28120 | 246 | NTP-binding protein L. paracasei subsp. paracasei ATCC 25302 | EEI67797.1 | 5.0E-71 |
EV3_034 | agtttggaagtgatgaaaacggtg | 28068-29459 | 463 | Putative helicase Lactobacilus phage A2 | CAB63670.1 | 1.0E-130 |
EV3_035 | acaaataggaga aaaatattatg | 29479-30042 | 187 | Single stranded binding protein L. hilgardii ATCC 8290 | EEI25831.1 | 4.0E-41 |
EV3_036 | aatatatgaaagggaaaatttatg | 30115-32442 | 775 | Phage primase, P4 family L. buchneri NRRL B-30929 | AEB73788.1 | 0.0 |
EV3_037 | taattttaaggagg aacacaaatg | 32745-33152 | 135 | Hypothetical protein | No hit | - |
EV3_038 | atgaaagatgtgatgtctgataatg | 33145-33465 | 106 | VRR-NUC domain Phage protein L. plantarum JDM1 | ACT61379.1 | 2.0E-33 |
EV3_039 | tgaatatagtaggag catttaaatg | 33462-33686 | 75 | Hypothetical protein | No hit | - |
EV3_040 | aagattgggagaaaataaccgtg | 33676-33849 | 58 | Hypothetical protein | No hit | - |
EV3_041 | caatgtaaggaag aatgataatg | 33872-34114 | 80 | Ribonucleoside-diphosphate reductase 2, Ent. faecalis | AAO80328.1 | 3.0E-13 |
EV3_042 | ataatcgtttcgttgggggttattatg | 34196-34612 | 138 | Phage transcriptional regulator Lactobacillus phage phig1e | CAA66778.1 | 3.0E-14 |
EV3_043 | taaacaaaaggagt agttaatatg | 34666-34833 | 56 | Putative transporter protein L. reuteri ATCC 53608 | CCC04545.1 | 3.0E-14 |