Skip to main content

Table 3 Summary of problem allele calls in arrays utilizing different quantities of starting DNA

From: Reproducibility and reliability of SNP analysis using human cellular DNA at or near nanogram levels

A. duplicate samples
Type of problems DNA quantity (ng)
  200 66.7 22.2 7.4
NC in both samples 1235 1313 1459 2077
NC in one sample, call in the other 1778 2212 2829 5319
Different calls in two samples 17 22 22 48
Total problem calls 3030 3547 4310 7444
Problem call % 0.42 0.49 0.59 1.02
B. triplicate samples
Type of problems DNA quantity (ng)   
  2.47 0.8   
NC in all samples 3022 5876   
NC in two samples, call in the third one 4618 11097   
NC in one sample, different calls in the other two 316 738   
NC in one sample, same calls in the other two 13304 29421   
Subtotal 18238 41256   
Calls in all three, 1 incorrect call* 199 391   
Calls in all three, 2 incorrect calls* 35 148   
Calls in all three, correctness uncertain* 6 4   
Subtotal 240 543   
Total problem calls 21500 47675   
Problem call % 3.00 6.58   
  1. *Correctness of the call is determined by the calls in the arrays using higher quantity of DNA samples.