Figure 5
From: Efficient transmission of Cassava brown streak disease viral pathogens by chip bud grafting
Reverse transcription polymerase chain reaction (RT-PCR) to detect CBSV in leaves of cassava plants 12 weeks after chip bud inoculation (A) TME204, (B) 60444 and (C) Ebwanateraka. Specific primers were used to detect 896 bp of the CBSV isolate Naliendele coat protein (CBSV-[TZ:Nal3-1:07]). Lanes are M: 1 kb + molecular marker; +: plasmid positive control; -: self-grafted, non-inoculated negative control; 1–6: CBSD symptomatic chip bud inoculated plants; 7–8: asymptomatic chip bud inoculated plants. Rubisco was run as a control.