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Table 2 Results of reference analysis using RefFinder [[21]] to evaluate the most stable reference across the study groups

From: Evaluation of long noncoding RNA MALAT1 as a candidate blood-based biomarker for the diagnosis of non-small cell lung cancer

Algorithm

Reference ranking

 

1 (most stable)

2

3 (least stable)

RefFinder (comprehensive ranking)

GM*

GAPDH

HPRT1

geNorm

GM* & GAPDH

 

HPRT1

NormFinder

GM*

GAPDH

HPRT1

BestKeeper

HPRT1

GM*

GAPDH

Comparative ΔCt method

GM*

GAPDH

HPRT1

  1. *Geometric mean (GM) was calculated from GAPDH and HPRT1.