Figure 2

Optimization of sonication protocols on the recovery of GAD. (a) Effect of cell concentration (% w/v) on GAD release by suspending the mycelia in 10 mL of citrate buffer at pH 5.5 and sonicated at 20 Khz, 50 W for 10 min; (b) Effect of buffer suspension volume (mL) on GAD release by sonicate 10% (w/v) of mycelia at 20 Khz, 50 W for 10 min; (c) Effect of acoustic power (watt) on GAD release by suspending mycelia (10% w/v) in 10 mL of citrate buffer at pH 5.5 and sonicated at 20 Khz for 10 min; (d) Effect of sonication time (min) on GAD release by suspending mycelia (10% w/v) in 10 mL of citrate buffer at pH 5.5 and sonicated at 20 Khz and 50 W. Results represent the mean (± SD) of three experiments.