Skip to main content

Table 2 Primer sequences used for multiplex PCR amplification panels

From: Three multiplex snapshot assays for SNP genotyping in candidate innate immune genes

Gene

SNP

(rs)

Forward

(5’→3’)

Reverse

(5’→3’)

Amplicon size (bp)

PCR mix (μM)

Panel 1

IL1-α

1800587

GCAATAGACCTTATGACACCTAAC

GGTAGAGAAGAGAACAGTGGTATT

731

3.0

IL1-β

16944

AGTATATGTGGGACAAAGTGGAAG

CTCATCTGGCATTGATCTGGTT

389

1.4

IL6

1800795

AGCCTGTTAATCTGGTCACTGA

CTTGTGGAGAAGGAGTTCATAGC

498

3.2

IL10

1800872

TTCTATGTGCTGGAGATGGTGTA

GGGTGGGCTAAATATCCTCAAAG

385

1.4

IL12

11575934

GGTTAAGTGACTGGTGCCAAG

TCCTGTACTCAGAGTGATCTTACC

210

3.0

Panel 2

TLR2

5743708

TGCTGCCATTCTCATTCTTCCTG

TCCTCAAATGACGGTACATCCA

282

1.4

IL10

1800896

ATCTGAAGAAGTCCTGATGTCACT

ACCATCTCCAGCACATAGAATGA

367

1.4

TLR2

4696480

CATAGTTGTCACAGTCCCTTGG

TGGCATGATCTCGGCTCAC

346

4.0

IL6

1800797

GGTGAAGAGACTCAGTGGCAAT

AGTGACCAGATTAACAGGCTAGAAT

444

2.0

TLR4

4986791

CCGATTAGCATACTTAGACTACTACCT

AGACTGGACAAGCCATTGAAGAT

555

3.2

Panel 3

IL12

401502

ACCTCTGTATGACATTGAGTAAGC

GAACCAACGGGACCACCAT

285

1.0

TLR2

121917864

CTGTGCTCTGTTCCTGCTGAT

GAAATGGGAGAAGTCCAGTTCATAC

382

1.0

TLR9

187084

ATTCATTCATTCAGCCTTCACTCAG

CCATCCAGCCTTCTTACAAACCT

324

1.0

CD14

2569190

GGCTTCCAGGCTTCACACT

AGGACACTGCCAGGAGACA

236

1.0

TLR4

4986790

TCCCTGAACCCTATGAACTTTATCC

AGGCTTGGTAGATCAACTTCTGAA

494

1.0

CD14

2569191

AAGGAAGGGATGGGACTATGTT

CAGGAATCTGAGGCAAGAGAAT

347

1.0