Table 3 Extension probes used for multiplex primer extension
From: Three multiplex snapshot assays for SNP genotyping in candidate innate immune genes
Gene | Positionchange | Variation | Extension probe (5’→3’) | Probe size (bp) | PCR mix (μM) |
|---|---|---|---|---|---|
Panel 1 | |||||
IL1-α | −889 | C>T | TTAATAATAGTAACCAGGCAACA | 23 | 2.0 |
IL1-β | −511 | A>G | TTTTTTTTTTGGTGCTGTTCTCTGCCTC | 28 | 1.2 |
IL6 | −174 | C>G | TTTTTTTTTTTTTTTCCCCTAGTTGTGTCTTGC | 33 | 2.0 |
IL10 | −592 | A>C | TTTTTTTTTTTTTTTTTTTTTCCTGTGACCCCGCCTGT | 38 | 1.2 |
IL12 | +705 | A>G | TTTTTTTTTTTTTTTTTTTTTTTTTTCCAGCTCCGACGACGGC | 43 | 2.0 |
Panel 2 | |||||
TLR2 | +2259 | A>G | TTTTTTTGCGCTTCTGCAAGCTGC | 24 | 0.8 |
IL10 | −1082 | A>G | TTTTTTTTCACTACTAAGGCTTCTTTGGGA | 30 | 0.8 |
TLR2 | −16933 | A>T | TTTTTTTTTTTTTTTTTATTGAAGGGCTGCATCTGG | 36 | 3.2 |
IL6 | −596 | A>G | TTTTTTTTTTTTTTTTTTTTTGTAACTGCACGAAATTTGAGG | 42 | 0.8 |
TLR4 | +1196 | C>T | TTTTTTTTTTTTTTTTTTTTTTTTGTTCTCAAAGTGATTTTGGGACAA | 48 | 3.2 |
Panel 3 | |||||
IL12 | +1196 | C>G | TTTTCAGGTGGCAAGGCCCC | 20 | 1.0 |
TLR2 | +2029 | C>T | TTTTTCCCTTCAAGTTGTGTCTTCATAAGC | 30 | 1.4 |
TLR9 | −1486 | C>T | TTTTTTTTTTTTTTCAGATAAAAGATCACTGCCCT | 35 | 1.0 |
CD14 | −260 | A>G | TTTTTTTTTTTTTTTTTTTTGGATGTTTCAGGGAGGGGGG | 40 | 1.0 |
TLR4 | +896 | A>G | TTTTTTTTTTTTTTTTTTTAGCATACTTAGACTACTACCTCGATG | 45 | 1.0 |
CD14 | −159 | C>T | TTTTTTTTTTTTTTTTTTTTTTTGAGGATTAATTAGTAACTCACCAGTTT | 50 | 2.0 |