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Figure 4 | BMC Research Notes

Figure 4

From: Evaluating real-time immunohistochemistry on multiple tissue samples, multiple targets and multiple antibody labeling methods

Figure 4

The impact of tissue thickness, size and position on RT-IHC data . A) 1, 3 and 9 nM 125I-labeled anti-HER2 antibody subsequently added in the same measurement at t = 0 h (1 nM), t = 20 h (3 nM) and t = 29 h (9 nM) to a dish containing SKOV3 xenograft (black) and mouse liver (grey), with high and low HER2 expression respectively. The antibody solution was replaced with PBS + 1% BSA (t = 44 h) in order to study the dissociation rate. B) 1 nM 125I-antibody binding to 10 μm (black solid line), 4 μm (black dotted line) and 2 μm (grey solid line) thick SKOV3 xenograft tissue slices. C) 1 nM 125I-antibody binding to SKOV3 xenograft tissues of different sizes: A full size tissue slice (black solid line), half a slice (black dotted line) or a quarter of a slice (grey solid line). D) 1 nM 125I-antibody binding to SKOV3 xenografts positioned approximately 0-16 mm (black solid line), 4-20 mm (black dotted line), 8-24 mm (grey solid line) or 12-28 mm (grey dotted line) from the rim of the dish. The detection area stretches from the rim and into approximately 20 mm of the dish. CPS = Counts per second.

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