Figure 2

Sequence alignment of different HBHA proteins. (A) Multiple sequence alignment was performed using the Clustal W program with the BLOSUM64 matrice allowing gaps (−). * indicate identical residues. : indicate conserved substitutions. ; indicate semi-conserved substitutions. Mtb, M. tuberculosis; BCG, M. bovis BCG; Mah, M. avium subsp. hominissuis and Map, M. avium subsp. paratuberculosis; Msmeg, M smegmatis. (B) Distribution of charges within the C-terminal domain of HBHA. The isoelectric point of each protein is indicated in brackets. Color code: Polar charged residues HKR (Pink); Polar residues GNQSTY (Green); Polar negative residues DE (Blue) and other residues (Red).