Heparin-Sepharose chromatography of recombinant map-HBHA produced in E. coli. A culture of recombinant E. coli producing map-HBHA was sonicated in 0.5 × PBS. The soluble material was loaded onto a heparin-Sepharose HiTrap column and eluted using a 0-1 M NaCl gradient. Whole cell lysate (WCL), flow-through material (FT) and eluted material were analyzed by SDS-PAGE. The fractions eluted between 100 and 350 mM NaCl contained map-HBHA as determined by HR-MS/MS. The molecular weights (MW) expressed in kDa are indicated in the left margin.