Figure 5
From: Purification of native HBHA from Mycobacterium avium subsp. paratuberculosis
Native map-HBHA purified by heparin-Sepharose chromatography in Tris–HCl buffer. Using a 20 mM Tris-HCl (pH 7.2) buffer a whole cell lysate (WCL) of Map was subjected to heparin-Sepharose HiTrap chromatography. SDS-PAGE analysis showed that the material eluted in fraction 11 at ca. 200 mM NaCl contains map-HBHA, as determined by HR-MS/MS. The map-Lbp/Hlp was identified in fraction 28, eluted at ca. 500 mM NaCl. The molecular weights (MW) expressed in kDa are indicated in the left margin.