Purification of native HBHA from Mycobacterium avium subsp. paratuberculosis

BMC Research Notes

Table 1 Identification of map-HBHA by mass spectrometry analysis

Peptides identified by HR-MS/MS	Peptide position start - end	Peptide modification
²A_acENPNIDDLR¹¹	2-11	Acetylated
⁶⁰FQEDLPEQFTELR⁷²	60-72
⁷³DKFTTEELR⁸¹	73-81
⁸²KAAEGYLEAATNR⁹⁴	82-94
⁸³AAEGYLEAATNR⁹⁴	83-94
¹⁰⁹LRSQTAFEDASAR¹²¹	109-121
¹¹¹SQTAFEDASAR¹²¹	111-121
¹¹¹SQTAFEDASARAEGYVDQAVELTQEALGTVASQTR ¹⁴⁵	111-145
¹²²AEGYVDQAVELTQEALGTVASQTR¹⁴⁵	122-145
¹⁵¹AAK_meLVGIELPGK¹⁶²	151-162	Methylated
¹⁵⁴LVGIELPGK¹⁶²	154-162
¹⁵⁴LVGIELPGKAEAAGK_me¹⁶⁸	154-168	Methylated
¹⁵⁴LVGIELPGKAEAAGKK¹⁶⁹	154-169
Peptides highlighted onto sequence^a

MA_acENPNIDDLR APLLAALGAADLALATVNDLIANLRERAEETRAETRTRVEERRARLTKFQEDLPEQFTELRDKFTTEELR KAAEGYLEAATNR YNELVERGEAALQRLRSQTAFEDASARAEGYVDQAVELTQEALGTVASQTR AVGERAAK_meLVGIELPGKAEAAGK_meK AQKAIAKAPAKKAPAKRVTQK.
^aThe 13 peptides identified by HR-MS/MS, text in italic, correspond to 52% of sequence coverage.

ISSN: 1756-0500