Gnomic organization, PCR- and Southern-profile of CyPA from P. indica . Genomic organization of CyPA gene from P. indica containing 10 exons and 9 introns. Introns spliced out and exons joined together and form 495 bp PiCyPA gene (A). Genomic organization of PiCyPA gene confirmed by PCR, lane 1 showing PCR with genomic DNA and lane 2 showing PCR with cDNA (B). Southern blotting confirmed single copy no of PiCyPA gene in lane 1 EcoRI used which is zero cutter and in lane 2 SacI used which is single cutter of PiCyPA gene (C).