Gene name | GenBank accession number | Primer sequence (5' → 3') | Amplicon length (bp) | Amplification efficiency (%) | aR2 | Average Cq of cDNA (n = 2) | ||||
---|---|---|---|---|---|---|---|---|---|---|
No culture | Control | LPS | LTA | Combined | ||||||
B2M | NM_213978 | F:ACTTTTCACACCGCTCCAGT R:CGGATGGAACCCAGATACAT | 180 | 89.20 | 0.992 | 20.4 ± 0.09 | 22.0 ± 0.63 | 32.2 ± 1.41 | 31.7 ± 1.39 | 30.38 ± 1.02 |
BLM | NM_001123084 | F:TCCTCACCTTCTGCATTTCC R:GTGGTGGCTGAGAATCCTGT | 152 | 93.12 | 0.993 | 26.5 ± 0.33 | 27.2 ± 0.79 | 35.2 ± 0.60 | 35.0 ± 0.72 | 35.7 ± 1.05 |
GAPDH | AF017079 | F:ACCCAGAAGACTGTGGATGG R:ACGCCTGCTTCACCACCTTC | 247 | 89.45 | 0.994 | 20.9 ± 0.78 | 23.5 ± 0.99 | 33.7 ± 0.79 | 33.5 ± 2.33 | 33.3 ± 1.34 |
HPRT1 | NM_001032376 | F:AACCTTGCTTTCCTTGGTCA R:TCAAGGGCATAGCCTACCAC | 150 | 91.88 | 0.997 | 24.7 ± 0.26 | 26.5 ± 1.01 | 34.8 ± 1.41 | 34.3 ± 0.82 | 34.0 ± 0.57 |
PPIA | NM_214353 | F:CACAAACGGTTCCCAGTTTT R:TGTCCACAGTCAGCAATGGT | 171 | 91.32 | 0.997 | 19.3 ± 0.19 | 20.4 ± 0.78 | 31.0 ± 1.46 | 31.2 ± 1.29 | 29.9 ± 0.78 |
RPL4 | DQ845176 | F:AGGAGGCTGTTCTGCTTCTG R:TCCAGGGATGTTTCTGAAGG | 185 | 90.21 | 0.993 | 19.8 ± 0.47 | 22.0 ± 0.79 | 31.9 ± 1.65 | 32.3 ± 1.67 | 30.7 ± 1.27 |
SDHA | DQ178128 | F:AGAGCCTCAAGTTCGGGAAG R:CAGGAGATCCAAGGCAAAAT | 149 | 92.24 | 0.996 | 25.6 ± 0.18 | 27.4 ± 0.53 | 34.7 ± 0.80 | 34.7 ± 0.85 | 34.7 ± 0.91 |
TBP | DQ178129 | F:ACGTTCGGTTTAGGTTGCAG R:GCAGCACAGTACGAGCAACT | 118 | 99.60 | 0.997 | 23.8 ± 0.13 | 24.7 ± 0.66 | 33.7 ± 2.61 | 32.4 ± 2.68 | 32.2 ± 0.45 |
YWHAZ | DQ178130 | F:ATTGGGTCTGGCCCTTAACT R:GCGTGCTGTCTTTGTATGACTC | 146 | 94.52 | 0.994 | 21.9 ± 0.25 | 23.9 ± 1.62 | 32.0 ± 1.29 | 32.7 ± 1.55 | 31.4 ± 0.94 |