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Table 1 Selected candidate reference genes, primers, and PCR reactions efficiencies

From: Evaluation of suitable reference genes for gene expression studies in porcine PBMCs in response to LPS and LTA

Gene name

GenBank accession number

Primer sequence (5' → 3')

Amplicon length (bp)

Amplification efficiency (%)

aR2

Average Cq of cDNA (n = 2)

No culture

Control

LPS

LTA

Combined

B2M

NM_213978

F:ACTTTTCACACCGCTCCAGT R:CGGATGGAACCCAGATACAT

180

89.20

0.992

20.4 ± 0.09

22.0 ± 0.63

32.2 ± 1.41

31.7 ± 1.39

30.38 ± 1.02

BLM

NM_001123084

F:TCCTCACCTTCTGCATTTCC R:GTGGTGGCTGAGAATCCTGT

152

93.12

0.993

26.5 ± 0.33

27.2 ± 0.79

35.2 ± 0.60

35.0 ± 0.72

35.7 ± 1.05

GAPDH

AF017079

F:ACCCAGAAGACTGTGGATGG R:ACGCCTGCTTCACCACCTTC

247

89.45

0.994

20.9 ± 0.78

23.5 ± 0.99

33.7 ± 0.79

33.5 ± 2.33

33.3 ± 1.34

HPRT1

NM_001032376

F:AACCTTGCTTTCCTTGGTCA R:TCAAGGGCATAGCCTACCAC

150

91.88

0.997

24.7 ± 0.26

26.5 ± 1.01

34.8 ± 1.41

34.3 ± 0.82

34.0 ± 0.57

PPIA

NM_214353

F:CACAAACGGTTCCCAGTTTT R:TGTCCACAGTCAGCAATGGT

171

91.32

0.997

19.3 ± 0.19

20.4 ± 0.78

31.0 ± 1.46

31.2 ± 1.29

29.9 ± 0.78

RPL4

DQ845176

F:AGGAGGCTGTTCTGCTTCTG R:TCCAGGGATGTTTCTGAAGG

185

90.21

0.993

19.8 ± 0.47

22.0 ± 0.79

31.9 ± 1.65

32.3 ± 1.67

30.7 ± 1.27

SDHA

DQ178128

F:AGAGCCTCAAGTTCGGGAAG R:CAGGAGATCCAAGGCAAAAT

149

92.24

0.996

25.6 ± 0.18

27.4 ± 0.53

34.7 ± 0.80

34.7 ± 0.85

34.7 ± 0.91

TBP

DQ178129

F:ACGTTCGGTTTAGGTTGCAG R:GCAGCACAGTACGAGCAACT

118

99.60

0.997

23.8 ± 0.13

24.7 ± 0.66

33.7 ± 2.61

32.4 ± 2.68

32.2 ± 0.45

YWHAZ

DQ178130

F:ATTGGGTCTGGCCCTTAACT R:GCGTGCTGTCTTTGTATGACTC

146

94.52

0.994

21.9 ± 0.25

23.9 ± 1.62

32.0 ± 1.29

32.7 ± 1.55

31.4 ± 0.94