Evaluation of six different DNA extraction methods for detection of Mycobacterium tuberculosis by means of PCR-IS6110: preliminary study

Table 4 Amplification after extraction of ten M. tuberculosis cultures

		DNA of tuberculosis cultures
		1 (PC)	2	3	4	5	6	7	8	9	10
Protocol	Concentrated	+	+	+	-	+	+	+	+	+	+
1	Dilution 1:10	+	+	+	-	+	+	+	-	+	+
	Dilution 1:100	-	+	+	-	+	+	+	+	+	-
	Dilution 1:1000	-	+	-	+	+	+	-	+	+	-
Protocol	Concentrated	+	+	+	-	+	+	+	+	+	+
2	Dilution 1:10	+	-	+	-	+	+	+	+	-	+
	Dilution1:100	+	-	+	+	+	+	+	+	-	+
	Dilution 1:1000	+	-	+	+	+	+	-	+	-	-
Protocol	Concentrated	+	+	+	+	+	+	+	+	-	+
3	Dilution 1:10	+	+	+	+	+	+	+	+	+	+
	Dilution 1:100	+	+	+	-	+	+	+	+	-	+
	Dilution 1:1000	+	+	+	-	+	+	+	+	+	+
Protocol	Concentrated	+	+	+	+	+	+	+	+	-	+
4	Dilution 1:10	+	+	+	-	+	+	+	+	-	+
	Dilution 1:100	+	+	+	-	-	+	+	+	-	+
	Dilution 1:1000	+	-	+	-	-	+	+	-	+	+
Protocol	Concentrated	+	+	+	+	+	+	+	+	+	+
5	Dilution 1:10	+	+	+	+	+	+	+	+	+	+
	Dilution 1:100	+	+	+	-	+	+	+	+	-	+
	Dilution 1:1000	+	-	-	-	+	+	-	-	-	-
Protocol	Concentrated	+	+	+	-	+	+	+	+	+	+
6	Dilution 1:10	+	+	+	+	+	+	+	+	+	+
6	Dilution 1:100	+	+	+	+	+	+	+	+	+	+
	Dilution 1:1000	+	+	+	-	-	+	-	+	+	+

ISSN: 1756-0500