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Table 5 Results of amplifications after extraction of DNA from five smears in sputum slides

From: Evaluation of six different DNA extraction methods for detection of Mycobacterium tuberculosis by means of PCR-IS6110: preliminary study

 

AFB AU

PCR

Culture

Sample 1

++

pos

M. tuberculosis

Sample 2

+

pos

M. tuberculosis

Sample 3

-

pos

M. tuberculosis

Sample 4

++

pos

M. tuberculosis

Sample 5

+

neg

M. kansasii

  1. + and ++ positives; pos = positive; neg = negative; PCR = Polymerase Chain Reaction; AFB AU = Acid-fast bacilli by Auramine.