A fast and efficient method for preparation of high-quality RNA from fungal mycelia

BMC Research Notes

Table 1 Purity of total RNA fractions isolated from Fusarium oxysporum f.sp. conglutinans and Rhizoctonia solani

	Biomass (g)	Yield (μg RNA/mg biomass)	²⁶⁰/₂₈₀	²⁶⁰/₂₃₀
			ratio	ratio
Fusarium oxysporum
static liquid culture	0.23^a	0.278	2.02	0.96
static liquid culture	0.17^a	0.215	1.98	0.98
shaken liquid culture	0.20^a	0.058	1.84	0.62
shaken liquid culture	0.20^a	0.051	1.82	0.50
solid media no overlay	0.24^b	0.094	1.86	0.26
solid media no overlay	0.51^b	0.093	1.74	0.37
membrane overlay	0.12	1.192	1.93	1.50
membrane overlay	0.11	1.362	1.93	1.56
membrane overlay (2 chloroform)	0.12	1.556	2.02	1.61
membrane overlay (2 chloroform)	0.12	1.684	2.00	1.50
Rhizoctonia solani
static liquid culture	0.38^a	0.324	1.97	0.63
static liquid culture	0.37^a	0.320	1.98	0.65
shaken liquid culture	0.28^a	0.934	1.97	0.95
shaken liquid culture	0.35^a	0.887	1.92	1.09
solid media no overlay	0.35^b	0.129	1.60	0.18
solid media no overlay	0.16^b	0.211	1.91	0.51
membrane overlay	0.20	0.845	1.83	0.59
membrane overlay	0.19	1.103	1.98	0.90
membrane overlay (2 chloroform)	0.19	1.110	2.00	0.71
membrane overlay (2 chloroform)	0.17	1.478	2.01	0.91

Measurements were performed using a Nanodrop reader.
^a Denotes samples where biomass measurements include residual water, all other samples are fresh weight. ^b Samples contained traces of agar, which resulted in gelatinous RNA pellets that were difficult to dissolve.

ISSN: 1756-0500