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Table 1 Purity of total RNA fractions isolated from Fusarium oxysporum f.sp. conglutinans and Rhizoctonia solani

From: A fast and efficient method for preparation of high-quality RNA from fungal mycelia

  Biomass (g) Yield (μg RNA/mg biomass) 260/280 260/230
    ratio ratio
Fusarium oxysporum     
static liquid culture 0.23a 0.278 2.02 0.96
static liquid culture 0.17a 0.215 1.98 0.98
shaken liquid culture 0.20a 0.058 1.84 0.62
shaken liquid culture 0.20a 0.051 1.82 0.50
solid media no overlay 0.24b 0.094 1.86 0.26
solid media no overlay 0.51b 0.093 1.74 0.37
membrane overlay 0.12 1.192 1.93 1.50
membrane overlay 0.11 1.362 1.93 1.56
membrane overlay (2 chloroform) 0.12 1.556 2.02 1.61
membrane overlay (2 chloroform) 0.12 1.684 2.00 1.50
Rhizoctonia solani     
static liquid culture 0.38a 0.324 1.97 0.63
static liquid culture 0.37a 0.320 1.98 0.65
shaken liquid culture 0.28a 0.934 1.97 0.95
shaken liquid culture 0.35a 0.887 1.92 1.09
solid media no overlay 0.35b 0.129 1.60 0.18
solid media no overlay 0.16b 0.211 1.91 0.51
membrane overlay 0.20 0.845 1.83 0.59
membrane overlay 0.19 1.103 1.98 0.90
membrane overlay (2 chloroform) 0.19 1.110 2.00 0.71
membrane overlay (2 chloroform) 0.17 1.478 2.01 0.91
  1. Measurements were performed using a Nanodrop reader.
  2. a Denotes samples where biomass measurements include residual water, all other samples are fresh weight. b Samples contained traces of agar, which resulted in gelatinous RNA pellets that were difficult to dissolve.