Isolation and characterization of nine polymorphic microsatellite markers for the deep-sea shrimp Nematocarcinus lanceopes (Crustacea: Decapoda: Caridea)

Table 1 Characteristics of nine polymorphic microsatellite loci for Nematocarcinus lanceopes tested on 55 individuals

Locus	Repeat motif	Oligonucleotide primer sequence 5' → 3'	Fragment length (bp)	N_A	H_O	H_E	F_{Null Alleles}
A06	(AG)25	Fw: GTCCTGAGTAATCGGCTCAGCTCT	187–233	17	0.927	0.924	0
		Rev: ACCCAGTTGGAAGCTGTTCTGAG
NL37	(AAT)19	Fw: GGGTTTAGGAGGAGTTTCGGGAC	186–228	12	0.782	0.832	0.017
		Rev: ACGAGTTGGAATGGGGCTGATG
NL16	(AG)12	Fw: TCAATTGTCCGGGACGCAAATGT	150–182	13	0.545	0.549	0
		Rev: AGTACATGGGCCACTAACTCCG
NL44	(ACAG)11	Fw: AATGGAGTGCAATGACGCTTGG	254–328	12	0.818	0.881	0.026
		Rev: TCGCAGTTTGTTTTAGAGGGAGC
NL51	(AT)12	Fw: TGATGACAGGGATTTGTCTTTCG	144–166	9	0.727	0.824	0.052
		Rev: TCCCCATTTGTACGCTATCC
NL53	(AC)11	Fw: ACAGTACACAGGCTACATAC	149–179	14	0.800	0.796	0.001
		Rev: ATCTTCATGTTATGCCCTCTAG
NL55	(AG)11	Fw: ACGCGAACAGTGCTAAGAAGAC	162–216	25	0.927	0.940	0
		Rev: CACCACAGCAAGGAACCTCC
NL56	(AG)12	Fw: AGTGAAAAGACTCAAATTCCTTGG	151–201	23	0.764*	0.934	0.077
		Rev: CATTACTGCTTCCTTCCTCTC
NL49	(AG)11	Fw: ACTCTACTTTGGCTTTCTCCCTC	155–183	11	0.927	0.861	0
		Rev: ACACGGGTCTTCCTGAGTGTTG

N_Anumber of alleles; H_Oobserved heterozygosity; H_Eexpected heterozygosity; F_{Null Alleles}frequency of null alleles.
* Indicates a locus that deviates from HWE after Bonferroni correction (9 comparisons; significance threshold = 0.005). Forward primers of the loci A06, NL44 and NL55 were labelled with a T7 tail (5’-TAATACGACTCACTATAG-3’) all other forward primers were labelled with a Sp6 tail (5’-GATTTAGGTGACACTAT-3’). A “pigtail” (5’-GTTTCTT-3’) was added to the reverse primers of the loci NL49, NL51, NL53, NL55 and NL56 to reduce stutter bands [16].

ISSN: 1756-0500