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Table 1 Primers used for RT-qPCR in the present study

From: Evaluation of reference genes for reverse transcription quantitative PCR analyses of fish-pathogenic Francisella strains exposed to different growth conditions

    

Primer efficiency

   

Target gene

Forward primer (5′- 3′)

Reverse primer (5′- 3′)

Amplicon size

Fn. ssp. noatunensis

Fn. ssp. orientalis

Gene ID

Position

uvrD

ACTATTTGTCGCGGGTCCTT

TCAAAGAAACGAAAACCTCCA

82 bp

2.050

1.959

12951493

596634-596715

rpoB

GTGGTAAAGCGCAATTTGGT

CAGCACCATATGCTTGTAACG

72 bp

1.986

1.988

12951517

620011-620082

gyrA

CGAGCTTTACGAGCTGCTTC

TCTTTTAGAGAACCCTAAAGAGGCT

87 bp

1.982

2.000

12952071

1187616-1187702

polA

AGCTGGAACTGGTCGTAATCA

ATCAGCATCTTCAGCAGCATA

82 bp

1.959

1.950

12951484

584274-584355

fopA

TACTGGTGCATGGGATGTTG

TCTTGGAGCCATTGTCTGAA

100 bp

1.902

1.938

12952182

1297252-1297351

ftsZ

TACCATACTCAGCGGCTTTC

GCGCCTGTAGTTGCTGAAGT

112 bp

1.986

1.997

12952792

136099-136210

ribC

ATCTCAACTAGCCACGCTCC

CGGTGGACACATGGTACAAG

87 bp

1.946

1.950

12952738

84136-84222

16S rRNA

AACGACTGTTAATACCGCATAATATCTG [17]

CCTTACCCTACCAACTAGCTAATCCA [17]

101 bp

1.954

1.954

12951375

463870-463970

iglC

TAGGCGTATAACACTGGCTGC

TGCTATAGAAGGCGGAGAGG

70 bp

2.006

1.905

12951826

930145-930214

  1. The complete genome of F. noatunensis ssp. orientalis strain Toba 04 (Accession number NC_017909), was used as a reference to assign the Gene ID.