|  |  |  | Primer efficiency |  |  |  |
---|---|---|---|---|---|---|---|
Target gene | Forward primer (5′- 3′) | Reverse primer (5′- 3′) | Amplicon size | Fn. ssp. noatunensis | Fn. ssp. orientalis | Gene ID | Position |
uvrD | ACTATTTGTCGCGGGTCCTT | TCAAAGAAACGAAAACCTCCA | 82 bp | 2.050 | 1.959 | 12951493 | 596634-596715 |
rpoB | GTGGTAAAGCGCAATTTGGT | CAGCACCATATGCTTGTAACG | 72 bp | 1.986 | 1.988 | 12951517 | 620011-620082 |
gyrA | CGAGCTTTACGAGCTGCTTC | TCTTTTAGAGAACCCTAAAGAGGCT | 87 bp | 1.982 | 2.000 | 12952071 | 1187616-1187702 |
polA | AGCTGGAACTGGTCGTAATCA | ATCAGCATCTTCAGCAGCATA | 82 bp | 1.959 | 1.950 | 12951484 | 584274-584355 |
fopA | TACTGGTGCATGGGATGTTG | TCTTGGAGCCATTGTCTGAA | 100 bp | 1.902 | 1.938 | 12952182 | 1297252-1297351 |
ftsZ | TACCATACTCAGCGGCTTTC | GCGCCTGTAGTTGCTGAAGT | 112 bp | 1.986 | 1.997 | 12952792 | 136099-136210 |
ribC | ATCTCAACTAGCCACGCTCC | CGGTGGACACATGGTACAAG | 87 bp | 1.946 | 1.950 | 12952738 | 84136-84222 |
16S rRNA | AACGACTGTTAATACCGCATAATATCTG [17] | CCTTACCCTACCAACTAGCTAATCCA [17] | 101 bp | 1.954 | 1.954 | 12951375 | 463870-463970 |
iglC | TAGGCGTATAACACTGGCTGC | TGCTATAGAAGGCGGAGAGG | 70 bp | 2.006 | 1.905 | 12951826 | 930145-930214 |