Optimization of a high-throughput whole blood expression profiling methodology and its application to assess the pharmacodynamics of interferon (IFN) beta-1a or polyethylene glycol-conjugated IFN beta-1a in healthy clinical trial subjects

Table 1 Whole blood transcripts most frequently upregulated by IFN beta-1a ^a

Gene Symbol	Affymetrix ID	Fold Change	P Value^b	LOD Score^c
USP18	219211_at	91.0	1.26E-17	29.6
SIGLEC1	219519_s_at	64.7	2.07E-14	22.3
SPATS2L	222154_s_at	27.2	1.57E-18	31.7
IFI44L	204439_at	26.5	2.54E-14	22.1
HERC5	219863_at	26.2	9.47E-18	29.9
SERPING1	200986_at	23.5	3.28E-12	17.3
RSAD2	213797_at	21.1	6.33E-12	16.7
IFI44	214059_at	20.5	2.70E-13	19.8
OAS3	218400_at	20.3	1.65E-14	22.5
OASL	205660_at	17.7	4.61E-16	26.1
IFIT1	203153_at	15.4	1.59E-13	20.3
RTP4	219684_at	14.6	1.35E-12	18.2
HERC6	219352_at	14.4	1.03E-19	34.4
IFIT3	204747_at	13.8	2.90E-14	22.0
OAS1	205552_s_at	13.3	7.93E-14	21.0
ISG15	205483_s_at	13.1	1.75E-14	22.5
MX1	202086_at	12.2	2.12E-13	20.0
DDX60	218986_s_at	11.4	9.23E-14	20.8
OAS2	204972_at	9.7	1.36E-11	15.9
LY6E	202145_at	7.0	1.80E-11	15.6

^aResults are from whole blood samples collected from healthy volunteers 6 hours post-dose with 30 μg IM IFN beta-1a. A composite IFN beta induction score was calculated from the geometric mean of the LOD score intensities of the top 20 transcripts.
^bValues were calculated using F-tests within the software package BRB Array Tools.
^cLOD score, logarithm (base 10) of odds score.

ISSN: 1756-0500