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Figure 2 | BMC Research Notes

Figure 2

From: Altering the selection capabilities of common cloning vectors via restriction enzyme mediated gene disruption

Figure 2

Strategies employed for cloning a tetracycline resistance cassette ( tetA ) via selectable marker restriction enzyme mediated gene disruption. The cassette was cloned into the resistance genes of pUC19, pZErO-2 and pET23a using unique restriction sites located in the selectable marker of each vector. Relevant restriction sites and features of each vector are indicated.

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