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Figure 3 | BMC Research Notes

Figure 3

From: Altering the selection capabilities of common cloning vectors via restriction enzyme mediated gene disruption

Figure 3

Restriction digest analysis of the vector pET23a: tetA carrying a foreign DNA segment. Cloning of a 590 bp foreign DNA was performed using Sac I and Hin dIII restriction sites located within the multiple cloning site of pET23a:tetA. Lane 1: 1 kb gene ruler DNA ladder (sizes as indicated in kb); lane 2: pET23a:tetA vector undigested; lane 3: pET23a:tetA, digested with Sac I and Hin dIII (5.01 kb); lane 4: pET23a:tetA, digested with Eco RV, a restriction site located within the tetA gene (5.02 kb); lane 5: pET23a:tetA vector carrying a 590 bp cloned DNA segment, undigested; lane 6: pET23a:tetA vector carrying a 590 bp cloned DNA segment, digested with Sac I and Hin dIII (0.59, 5.01 kb); lane 7: pET23a:tetA vector containing cloned DNA, digested with Eco RV, a restriction site located within both the tetA gene and the cloned DNA (3.30, 2.30 kb).

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