Figure 3From: Altering the selection capabilities of common cloning vectors via restriction enzyme mediated gene disruptionRestriction digest analysis of the vector pET23a: tetA carrying a foreign DNA segment. Cloning of a 590Â bp foreign DNA was performed using Sac I and Hin dIII restriction sites located within the multiple cloning site of pET23a:tetA. Lane 1: 1Â kb gene ruler DNA ladder (sizes as indicated in kb); lane 2: pET23a:tetA vector undigested; lane 3: pET23a:tetA, digested with Sac I and Hin dIII (5.01Â kb); lane 4: pET23a:tetA, digested with Eco RV, a restriction site located within the tetA gene (5.02Â kb); lane 5: pET23a:tetA vector carrying a 590Â bp cloned DNA segment, undigested; lane 6: pET23a:tetA vector carrying a 590Â bp cloned DNA segment, digested with Sac I and Hin dIII (0.59, 5.01Â kb); lane 7: pET23a:tetA vector containing cloned DNA, digested with Eco RV, a restriction site located within both the tetA gene and the cloned DNA (3.30, 2.30Â kb).Back to article page