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Table 2 Viable transformant counts of E. coli transformants carrying vectors with an undisrupted or disrupted ampicillin selectable marker

From: Altering the selection capabilities of common cloning vectors via restriction enzyme mediated gene disruption

 

Viable transformant count (cfu/ml) on medium

Transformant

LB

LB + Amp

LB + Tet

pUC19

2.22 × 109

2.25 × 109

0

pUC19:tetA

4.2 × 109

0

2.85 × 109

pET23a

2.85 × 109

2.39 × 109

0

pET23a:tetA

2.03 × 109

0

2.11 × 109

  1. Disruptants were created by cloning a tetracycline selectable marker gene into a unique restriction site in the vector encoded ampicillin resistance gene. Transformants were plated onto LB medium supplemented with either ampicillin or tetracycline. cfu; colony forming unit.