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Table 2 Viable transformant counts of E. coli transformants carrying vectors with an undisrupted or disrupted ampicillin selectable marker

From: Altering the selection capabilities of common cloning vectors via restriction enzyme mediated gene disruption

  Viable transformant count (cfu/ml) on medium
Transformant LB LB + Amp LB + Tet
pUC19 2.22 × 109 2.25 × 109 0
pUC19:tetA 4.2 × 109 0 2.85 × 109
pET23a 2.85 × 109 2.39 × 109 0
pET23a:tetA 2.03 × 109 0 2.11 × 109
  1. Disruptants were created by cloning a tetracycline selectable marker gene into a unique restriction site in the vector encoded ampicillin resistance gene. Transformants were plated onto LB medium supplemented with either ampicillin or tetracycline. cfu; colony forming unit.