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Table 3 Viable transformant counts of E. coli transformants carrying vectors with an undisrupted or disrupted kanamycin selectable marker

From: Altering the selection capabilities of common cloning vectors via restriction enzyme mediated gene disruption

 

Viable transformant count (cfu/ml) on medium

Transformant

LB

LB + Kan

LB + Tet

pZErO

2.05 × 109

1.35 × 109

0

pZErO:tetA

1.06 × 107

0

1.07 × 107

  1. Disruptants were created by cloning a tetracycline selectable marker gene into a unique restriction site in the vector encoded kanamycin resistance gene. Transformants were plated onto LB medium supplemented with either kanamycin or tetracycline. cfu; colony forming unit.
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BMC Research Notes

ISSN: 1756-0500

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