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Table 4 Viable transformant counts of E. coli transformants carrying vectors with an undisrupted or disrupted ampicillin selectable marker

From: Altering the selection capabilities of common cloning vectors via restriction enzyme mediated gene disruption

 

Viable transformant count (cfu/ml) on medium

Transformant

LB

LB + Amp

LB + Kan

pUC19

2.22 × 109

2.25 × 109

0

pUC19:kanR

6.7 × 108

0

7.3 × 108

pBlueScript

2.87 × 109

2.81 × 109

0

pBlueScript:kanR

2.05 × 109

0

1.78 × 109

  1. Disruptants were created by cloning a kanamycin selectable marker gene into a unique restriction site in the vector encoded ampicillin resistance gene. Transformants were plated onto LB medium supplemented with either ampicillin or kanamycin. cfu; colony forming unit.