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Table 4 Viable transformant counts of E. coli transformants carrying vectors with an undisrupted or disrupted ampicillin selectable marker

From: Altering the selection capabilities of common cloning vectors via restriction enzyme mediated gene disruption

  Viable transformant count (cfu/ml) on medium
Transformant LB LB + Amp LB + Kan
pUC19 2.22 × 109 2.25 × 109 0
pUC19:kanR 6.7 × 108 0 7.3 × 108
pBlueScript 2.87 × 109 2.81 × 109 0
pBlueScript:kanR 2.05 × 109 0 1.78 × 109
  1. Disruptants were created by cloning a kanamycin selectable marker gene into a unique restriction site in the vector encoded ampicillin resistance gene. Transformants were plated onto LB medium supplemented with either ampicillin or kanamycin. cfu; colony forming unit.